In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration.
- How do you use a calibration curve in chemistry?
- What is calibration in chemistry?
- Is a calibration curve relate to Beer's Law?
- What is a calibration curve absorbance?
How do you use a calibration curve in chemistry?
The curve is constructed by measuring the concentration and absorbance of several prepared solutions, called calibration standards. Once the curve has been plotted, the concentration of the unknown solution can be determined by placing it on the curve based on its absorbance or other observable variable.
What is calibration in chemistry?
1.1.
In analytical chemistry, calibration is defined as the process of assessment and refinement of the accuracy and precision of a method, and particularly the associated measuring equipment (i.e., an instrument), employed for the quantitative determination of a sought-after analyte [2].
Is a calibration curve relate to Beer's Law?
Equation 8.2. 3 and equation 8.2. 4, which establish the linear relationship between absorbance and concentration, are known as Beer's law. Calibration curves based on Beer's law are common in quantitative analyses.
What is a calibration curve absorbance?
A calibration curve is a standard graph which shows the change in the response of an analytical instrument towards different concentrations of the analyte. It indicates absorbance in y-axis and concentration in x-axis. Absorbance is the response of a spectrophotometer towards the concentration of a sample.